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differentiation 36  (R&D Systems)


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    R&D Systems differentiation 36
    Differentiation 36, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+cd36+fc/Recombinant+Human+CD36%2FSR-B3+Fc+Chimera+Protein%2C+CF/pmc13064270-68-11-24
    Average 94 stars, based on 14 article reviews
    differentiation 36 - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: The GPI sidechain of Toxoplasma gondii prevents parasite pathogenesis
    Article Snippet: After two further washes, the samples were analyzed using a ZE5 Cell Analyzer (Bio-Rad) and data processed as described above. .. 10 7 parasites were incubated with 0.5 μg recombinant human IgG Fc (R&D Systems, cat# 110-HG) or recombinant CD36-Fc (R&D Systems, #2519-CD) in 50μl binding buffer (0.14 M NaCl, 2.5mM CaCl 2 , 0.01M HEPES (pH7.4), 3% BSA) for 1 hr at 15°C. .. Parasites were washed once to remove unbound recombinant protein and the parasites were then incubated with mouse anti-SAG1-AF405 (TP3cc, Novus Biologicals) and donkey anti-human IgG-Daylight 550 (Thermo Fisher, SA5-10127).

    Article Title: The GPI sidechain of Toxoplasma gondii inhibits parasite pathogenesis
    Article Snippet: After two further washes, the samples were analyzed using a ZE5 Cell Analyzer (Bio-Rad), and data were processed as described above. .. Parasites (10 7 ) were incubated with 0.5 μg recombinant human IgG Fc (R&D Systems # 110-HG) or recombinant CD36-Fc (R&D Systems, #2519 CD) in 50 μL binding buffer (0.14 M NaCl, 2.5 mM CaCl 2 , 0.01 M HEPES [pH 7.4], 3% BSA) for 1 hour at 15°C. .. Parasites were washed once to remove unbound recombinant protein and the parasites were then incubated with mouse anti-SAG1-AF405 (TP3cc, Novus Biologicals) and donkey anti-human IgG-Daylight 550 (Thermo Fisher, SA5-10127).

    Recombinant:

    Article Title: The GPI sidechain of Toxoplasma gondii prevents parasite pathogenesis
    Article Snippet: After two further washes, the samples were analyzed using a ZE5 Cell Analyzer (Bio-Rad) and data processed as described above. .. 10 7 parasites were incubated with 0.5 μg recombinant human IgG Fc (R&D Systems, cat# 110-HG) or recombinant CD36-Fc (R&D Systems, #2519-CD) in 50μl binding buffer (0.14 M NaCl, 2.5mM CaCl 2 , 0.01M HEPES (pH7.4), 3% BSA) for 1 hr at 15°C. .. Parasites were washed once to remove unbound recombinant protein and the parasites were then incubated with mouse anti-SAG1-AF405 (TP3cc, Novus Biologicals) and donkey anti-human IgG-Daylight 550 (Thermo Fisher, SA5-10127).

    Article Title: The GPI sidechain of Toxoplasma gondii inhibits parasite pathogenesis
    Article Snippet: After two further washes, the samples were analyzed using a ZE5 Cell Analyzer (Bio-Rad), and data were processed as described above. .. Parasites (10 7 ) were incubated with 0.5 μg recombinant human IgG Fc (R&D Systems # 110-HG) or recombinant CD36-Fc (R&D Systems, #2519 CD) in 50 μL binding buffer (0.14 M NaCl, 2.5 mM CaCl 2 , 0.01 M HEPES [pH 7.4], 3% BSA) for 1 hour at 15°C. .. Parasites were washed once to remove unbound recombinant protein and the parasites were then incubated with mouse anti-SAG1-AF405 (TP3cc, Novus Biologicals) and donkey anti-human IgG-Daylight 550 (Thermo Fisher, SA5-10127).

    Binding Assay:

    Article Title: The GPI sidechain of Toxoplasma gondii prevents parasite pathogenesis
    Article Snippet: After two further washes, the samples were analyzed using a ZE5 Cell Analyzer (Bio-Rad) and data processed as described above. .. 10 7 parasites were incubated with 0.5 μg recombinant human IgG Fc (R&D Systems, cat# 110-HG) or recombinant CD36-Fc (R&D Systems, #2519-CD) in 50μl binding buffer (0.14 M NaCl, 2.5mM CaCl 2 , 0.01M HEPES (pH7.4), 3% BSA) for 1 hr at 15°C. .. Parasites were washed once to remove unbound recombinant protein and the parasites were then incubated with mouse anti-SAG1-AF405 (TP3cc, Novus Biologicals) and donkey anti-human IgG-Daylight 550 (Thermo Fisher, SA5-10127).

    Article Title: The GPI sidechain of Toxoplasma gondii inhibits parasite pathogenesis
    Article Snippet: After two further washes, the samples were analyzed using a ZE5 Cell Analyzer (Bio-Rad), and data were processed as described above. .. Parasites (10 7 ) were incubated with 0.5 μg recombinant human IgG Fc (R&D Systems # 110-HG) or recombinant CD36-Fc (R&D Systems, #2519 CD) in 50 μL binding buffer (0.14 M NaCl, 2.5 mM CaCl 2 , 0.01 M HEPES [pH 7.4], 3% BSA) for 1 hour at 15°C. .. Parasites were washed once to remove unbound recombinant protein and the parasites were then incubated with mouse anti-SAG1-AF405 (TP3cc, Novus Biologicals) and donkey anti-human IgG-Daylight 550 (Thermo Fisher, SA5-10127).



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    Fig. 4. C1s inhibits binding of IT4var20 IE to EPCR and IT4var13 IE binding to <t>CD36</t> but not ICAM-1. (A) Binding of IT4var20 IEs to EPCR in the absence or presence of C1s (100 μg/mL). Recombinant EPCR (rEPCR) was included as a control and indicator of how well C1s treatment abrogated IT4var20 binding to EPCR. (B) Binding of IT4var13 IEs to CD36 or ICAM-1 in the absence or presence of C1s (100 μg/mL). In both binding assays, bovine serum albumin (BSA) was included as a negative control. The mean and SEM are shown, and each data point is from an independent experiment. For statistical testing, all mean values were log-transformed, and a two-tailed unpaired t test was conducted to compare experimental groups to the positive control (EPCR only). (C) Var gene transcript levels relative to the housekeeping control gene, seryl-t-transferase, confirming the major var types being expressed as IT4var20 and IT4var13. *P < 0.05; **P < 0.01; ns, not significant.
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    Fig. 4. C1s inhibits binding of IT4var20 IE to EPCR and IT4var13 IE binding to <t>CD36</t> but not ICAM-1. (A) Binding of IT4var20 IEs to EPCR in the absence or presence of C1s (100 μg/mL). Recombinant EPCR (rEPCR) was included as a control and indicator of how well C1s treatment abrogated IT4var20 binding to EPCR. (B) Binding of IT4var13 IEs to CD36 or ICAM-1 in the absence or presence of C1s (100 μg/mL). In both binding assays, bovine serum albumin (BSA) was included as a negative control. The mean and SEM are shown, and each data point is from an independent experiment. For statistical testing, all mean values were log-transformed, and a two-tailed unpaired t test was conducted to compare experimental groups to the positive control (EPCR only). (C) Var gene transcript levels relative to the housekeeping control gene, seryl-t-transferase, confirming the major var types being expressed as IT4var20 and IT4var13. *P < 0.05; **P < 0.01; ns, not significant.
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    Fig. 4. C1s inhibits binding of IT4var20 IE to EPCR and IT4var13 IE binding to <t>CD36</t> but not ICAM-1. (A) Binding of IT4var20 IEs to EPCR in the absence or presence of C1s (100 μg/mL). Recombinant EPCR (rEPCR) was included as a control and indicator of how well C1s treatment abrogated IT4var20 binding to EPCR. (B) Binding of IT4var13 IEs to CD36 or ICAM-1 in the absence or presence of C1s (100 μg/mL). In both binding assays, bovine serum albumin (BSA) was included as a negative control. The mean and SEM are shown, and each data point is from an independent experiment. For statistical testing, all mean values were log-transformed, and a two-tailed unpaired t test was conducted to compare experimental groups to the positive control (EPCR only). (C) Var gene transcript levels relative to the housekeeping control gene, seryl-t-transferase, confirming the major var types being expressed as IT4var20 and IT4var13. *P < 0.05; **P < 0.01; ns, not significant.
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    Fig. 4. C1s inhibits binding of IT4var20 IE to EPCR and IT4var13 IE binding to <t>CD36</t> but not ICAM-1. (A) Binding of IT4var20 IEs to EPCR in the absence or presence of C1s (100 μg/mL). Recombinant EPCR (rEPCR) was included as a control and indicator of how well C1s treatment abrogated IT4var20 binding to EPCR. (B) Binding of IT4var13 IEs to CD36 or ICAM-1 in the absence or presence of C1s (100 μg/mL). In both binding assays, bovine serum albumin (BSA) was included as a negative control. The mean and SEM are shown, and each data point is from an independent experiment. For statistical testing, all mean values were log-transformed, and a two-tailed unpaired t test was conducted to compare experimental groups to the positive control (EPCR only). (C) Var gene transcript levels relative to the housekeeping control gene, seryl-t-transferase, confirming the major var types being expressed as IT4var20 and IT4var13. *P < 0.05; **P < 0.01; ns, not significant.
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    Fig. 4. C1s inhibits binding of IT4var20 IE to EPCR and IT4var13 IE binding to CD36 but not ICAM-1. (A) Binding of IT4var20 IEs to EPCR in the absence or presence of C1s (100 μg/mL). Recombinant EPCR (rEPCR) was included as a control and indicator of how well C1s treatment abrogated IT4var20 binding to EPCR. (B) Binding of IT4var13 IEs to CD36 or ICAM-1 in the absence or presence of C1s (100 μg/mL). In both binding assays, bovine serum albumin (BSA) was included as a negative control. The mean and SEM are shown, and each data point is from an independent experiment. For statistical testing, all mean values were log-transformed, and a two-tailed unpaired t test was conducted to compare experimental groups to the positive control (EPCR only). (C) Var gene transcript levels relative to the housekeeping control gene, seryl-t-transferase, confirming the major var types being expressed as IT4var20 and IT4var13. *P < 0.05; **P < 0.01; ns, not significant.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Complement C1s cleaves PfEMP1 at interdomain conserved sites inhibiting Plasmodium falciparum cytoadherence.

    doi: 10.1073/pnas.2104166118

    Figure Lengend Snippet: Fig. 4. C1s inhibits binding of IT4var20 IE to EPCR and IT4var13 IE binding to CD36 but not ICAM-1. (A) Binding of IT4var20 IEs to EPCR in the absence or presence of C1s (100 μg/mL). Recombinant EPCR (rEPCR) was included as a control and indicator of how well C1s treatment abrogated IT4var20 binding to EPCR. (B) Binding of IT4var13 IEs to CD36 or ICAM-1 in the absence or presence of C1s (100 μg/mL). In both binding assays, bovine serum albumin (BSA) was included as a negative control. The mean and SEM are shown, and each data point is from an independent experiment. For statistical testing, all mean values were log-transformed, and a two-tailed unpaired t test was conducted to compare experimental groups to the positive control (EPCR only). (C) Var gene transcript levels relative to the housekeeping control gene, seryl-t-transferase, confirming the major var types being expressed as IT4var20 and IT4var13. *P < 0.05; **P < 0.01; ns, not significant.

    Article Snippet: Plastic plates were spotted with 5 μL of EPCR (13320-H02H; Sino Biologicals), CSA (a gift from Dr. Michal Fried, NIAID/NIH, Bethesda, MD), CD36 (1955-CD; R&D Systems), or ICAM-1 (720-IC; R&D Systems); all recombinant proteins, except for ICAM-1 which was used at 100 μg/mL, were at a concentration of 50 μg/mL.

    Techniques: Binding Assay, Recombinant, Control, Negative Control, Transformation Assay, Two Tailed Test, Positive Control

    Fig. 6. Analysis of C1s-treated recombinant PfEMP1. (A) SDS/PAGE gel showing two representative PfEMP1 CIDR domains (EPCR-binding IT4var19 CIDRα1 and CD36-binding CIDRα3) and two EPCR-binding head-structure domain complexes (PFD1235w [NTSA-DBLα1-CIDRα1] and IT4var20 [NTSA-DBLα1-CIDRα1]) before and after treatment with 50 nM C1s at 1:1 molar ratio for 1 h at 37 °C. No proteolysis was seen. (B) ELISA data showing the effect of 50 nM C1s pretreatment on binding of recombinant PfEMP1 proteins to coated EPCR, CD36, or ICAM-1 (filled boxes) versus EPCR binding to coated recombinant PfEMP1 (hashed boxes). Whereas C1s treatment reduced binding activity of full-length PfEMP1 ectodomains, recombinant PfEMP1 head structure domains are insensitive to C1s treatment, indicating that C1s cleavage sites are located in the interdomain regions, consistent with MS analysis. As a control, C1s treatment of EPCR did not affect its binding to full-length PfEMP1 ectodomains (hashed boxes).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Complement C1s cleaves PfEMP1 at interdomain conserved sites inhibiting Plasmodium falciparum cytoadherence.

    doi: 10.1073/pnas.2104166118

    Figure Lengend Snippet: Fig. 6. Analysis of C1s-treated recombinant PfEMP1. (A) SDS/PAGE gel showing two representative PfEMP1 CIDR domains (EPCR-binding IT4var19 CIDRα1 and CD36-binding CIDRα3) and two EPCR-binding head-structure domain complexes (PFD1235w [NTSA-DBLα1-CIDRα1] and IT4var20 [NTSA-DBLα1-CIDRα1]) before and after treatment with 50 nM C1s at 1:1 molar ratio for 1 h at 37 °C. No proteolysis was seen. (B) ELISA data showing the effect of 50 nM C1s pretreatment on binding of recombinant PfEMP1 proteins to coated EPCR, CD36, or ICAM-1 (filled boxes) versus EPCR binding to coated recombinant PfEMP1 (hashed boxes). Whereas C1s treatment reduced binding activity of full-length PfEMP1 ectodomains, recombinant PfEMP1 head structure domains are insensitive to C1s treatment, indicating that C1s cleavage sites are located in the interdomain regions, consistent with MS analysis. As a control, C1s treatment of EPCR did not affect its binding to full-length PfEMP1 ectodomains (hashed boxes).

    Article Snippet: Plastic plates were spotted with 5 μL of EPCR (13320-H02H; Sino Biologicals), CSA (a gift from Dr. Michal Fried, NIAID/NIH, Bethesda, MD), CD36 (1955-CD; R&D Systems), or ICAM-1 (720-IC; R&D Systems); all recombinant proteins, except for ICAM-1 which was used at 100 μg/mL, were at a concentration of 50 μg/mL.

    Techniques: Recombinant, SDS Page, Binding Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Control